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Lister AS, Dorsey JG, Burton DE (1997) J High Resolut Chromatogr 20:523 144. Roed L, Lundanes E, Greibrokk T (1999) Electrophoresis 20:2373 145. Smith NW (2000) J Chromatogr A 887:233 146. Tobler E, Lämmerhofer M, Lindner W (2000) J Chromatogr A 875:341 147. Walhagen K, Unger KK, Hearn MTW (2000) J Chromatogr A 887:165 148. Dittmann MM, Masuch K, Rozing GP (2000) J Chromatogr A 887:209 149. Peters EC, Petro M, Svec F, Fréchet JMJ (1998) Anal Chem 70:2296 150. Altria KD, Smith NW, Turnbull CH (1998) J Chromatogr B 717:341 151.

Hjertén S, Eaker D, Elenbring K, Ericson C, Kubo K, Liao JL, Zeng CM, Lindström PA, Lindh C, Palm A, Srichiayo T, Valcheva L, Zhang R (1995) Jpn J Electrophoresis 39:105 115. Ericson C, Liao JL, Nakazato K, Hjertén S (1997) J Chromatogr 767:33 116. Liao JL, Chen N, Ericson C, Hjertén S (1996) Anal Chem 68:3468 117. Ericson C, Hjertén S (1999) Anal Chem 71:1621 118. Hoegger D, Freitag R (2001) J Chromatogr A 914:211 119. Palm A, Novotny MV (1997) Anal Chem 69:4499 120. Que AH, Palm A, Baker AG, Novotny MV (2000) J Chromatogr A 887:379 121.

Therefore, much faster separations are possible and the productivity of chromatographic processes can be increased by at least one order of magnitude as compared to traditional chromatographic columns packed with porous particles. Besides the speed, the nature of the pores allows easy access even in the case of large molecules, which make monolithic supports a method of choice for the separation of nanoparticles like pDNA and viruses. Finally, for the optimal purification of larger biomolecules, the chromatographic column needs to be short.

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Advances In Biochemical Engineering - Biotechnology - Modern Adva


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