By Bhakti Bajpai (auth.), Indu Ravi, Mamta Baunthiyal, Jyoti Saxena (eds.)
The e-book “Advances in Biotechnology” is set fresh advances in many of the vital fields which are ongoing in convinced biotechnological purposes. Biotechnology has been relatively important in protecting velocity with the calls for of each expanding human inhabitants and in enhancing the standard of human lifestyles. significant biotechnological achievements linked to human welfare were from the fields like genetic engineering; transgenic vegetation and animals; genomics, proteomics, monoclonal antibodies for the analysis of disorder, gene remedy and so forth. Fourteen authoritative chapters written via specialists having event in lecturers and examine on present advancements and destiny traits in biotechnology were empathized. The e-book offers an in depth account of assorted methodologies utilized in biotechnology i.e. excessive capability vectors, DNA sequencing facing subsequent new release sequencing, Molecular markers, DNA microarray expertise, in addition to Proteomics that experience revolutionized biotechnology with a big selection of purposes. The publication not just provides a well-founded rationalization of the themes but in addition goals to give up to date experiences of present examine efforts, a few considerate discussions at the strength advantages and dangers taken with generating biotechnological items and the demanding situations of bringing such items to marketplace. it's going to turn out to be a very good reference paintings for either academicians and researchers, indicating new beginning issues to younger researchers for brand spanking new initiatives within the box. The booklet is meant for biotechnologist, biologist, researchers, lecturers and scholars of Biosciences and Biotechnology.
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Extra info for Advances in Biotechnology
More recently, the RAD tag isolation procedure has been 37 modified for use with high-throughput sequencing on the Illumina platform. In addition, Miller et al. (2007b) demonstrated that RAD markers, using microarray platform, allowed highthroughput, high-resolution genotyping in both model and non-model systems. 3 Single Feature Polymorphism A third high-throughput method is single feature polymorphism (SFP) which is done by labeling genomic DNA (target) and hybridizing to arrayed oligonucleotide probes that are complementary to insert-delete (INDEL) loci.
2198 Maxam AM, Gilbert W (1977) A new method for sequencing DNA. Proc Nat Acad Sci 74(2):560–564. PMCID: PMC392330. 560 Nakamura K, Oshima T, Morimoto T, Ikeda S, Yoshikawa H, Shiwa Y, Ishikawa S, Linak MC, Hirai A, Takahashi H, Altaf-Ul-Amin M, Ogasawara N, Kanaya S (2011) Sequence-specific error profile of Illumina sequencers. Nucleic Acids Res 39(13). 1093/nar/gkr344 Pettersson E, Lundeberg J, Ahmadian A (2009) Generations of sequencing technologies. Genomics 93(2):105–11. 003 Porreca JG (2010) Genome sequencing on nanoballs.
Knowledge of nucleotide sequence is used to design specific primers (generally 20–25 bp) for amplification of the microsatellite region along with flanking sequences by PCR. Such amplified amplicon is then identified by construction of a small-insert genomic library, followed by screening the library with a synthetically labeled oligonucleotide probe. Positive clones thus obtained are sequenced for detection and confirmation of polymorphism. Microsatellite polymorphism of organism whose genome is not sequenced may also be identified by screening sequence databases of closely related species for microsatellite sequence motifs.
Advances in Biotechnology by Bhakti Bajpai (auth.), Indu Ravi, Mamta Baunthiyal, Jyoti Saxena (eds.)