By Jack F Kay
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Extra resources for Analyses for hormonal substances in food producing animals
A change in the type of sire used in the national dairy herd. The point was also raised that, if we 38 Chapter 1 were to continue to exclude growth promoters on the grounds that it would raise the exposure of hormones to the consumer, then logically one should consider excluding meat from pregnant animals and possibly those in oestrus. JECFA15 concluded in their evaluation of the numerous studies using authorised doses of the three natural steroids either alone or in combination that the hormone concentrations in edible tissues and blood were sometimes statistically signiﬁcantly higher than the corresponding values found in concurrent controls but were always within the physiological range of these substances in bovine animals.
A further study considered by the SCVPH involved the interaction of hormonal substances and their metabolites with sex hormone-binding globulin (SHBG) or the analogous sex hormone-binding protein (SBP) from trout plasma. Zeranol and its metabolites were included in this study, and were found to have low binding aﬃnity to these proteins; this would result in high bioavailability when present in plasma, but also fairly rapid metabolism. This unpublished study is discussed further. 4 Melengestrol Acetate At the time of the SCVPH 1999 Opinion, the information available on melengestrol acetate (MGA) was sparse.
However, the study was unable to conﬁrm the possible foetal nature of these cells using a battery of speciﬁc markers as none of the available antibodies worked on rabbit tissues. Evidence for eﬀects of TBA and zeranol on gonocyte development in the foetal testis was provided by increased numbers of these The Use of Hormomally Active Substances 31 cells being immunopositive for PG-2, but as the role of this antigen is unknown, it is not possible to evaluate the signiﬁcance of this observation (which was based on only two animals per group).
Analyses for hormonal substances in food producing animals by Jack F Kay